Composite
Part:BBa_K2284010:Design
Designed by: Eleanor Rose Boardman Group: iGEM17_UNOTT (2017-10-26)
P5 targetable RFP reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 608
Illegal AgeI site found at 720 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Constructed using BsaI sites to minimise restriction sites in the sequence
Source
Synthetic promoter, RFP from kit plate, and synthesised terminator.